ADSORPTION IMMOBILIZATION OF MICROORGANISM CELLS ON MINERAL CARRIERS

Abstract

This paper presents the results of laboratory work to determine the effectiveness of adsorbing immobilization of natural minerals as carriers of destructor microorganisms. A consortium of microorganisms of Dietzia maris strains was immobilized on the minerals haydite and zeolite. The problem of eliminating oil pollution from soil surfaces has important economic and environmental significance due to increased emergency situations in hydrocarbon extraction areas. The controlled biological method of bioremediation using immobilized microorganisms offers a cost-effective solution for removing petroleum products to background levels. The experimental methodology involved preparing mineral carriers (zeolite and haydite), cultivating a consortium of Dietzia maris strains (U2.1, U2.6, MB3, KU1), and measuring adsorption capacity through spectrophotometric analysis. According to the calculated results, zeolite demonstrated superior adsorption capacity with 19.69 mg of dry cells per gram of carrier, while haydite showed 17.92 mg per gram. The effectiveness of adsorption depends on properties of the microorganism, sorbent characteristics, and environmental conditions. This experiment demonstrated that zeolite sorbent enables high efficiency in biological remediation of hydrocarbons, making it an effective biocatalyst for petroleum hydrocarbon bioremediation processes. Natural minerals available in Kazakhstan, particularly zeolite, represent promising and economically viable carriers for immobilized microorganisms in oil-contaminated soil remediation.